Antonios Koutroupis begun to study Byzantine Music at the age of 11 and attended Byzantine Music Academy in Cyprus. He graduated from the State Conservatory of St Petersburg. Continued his vocal perfection in Italy where he was the precentor of the Greek Cathedral church of St George in Venice. Was invited to teach and sing Byzantine Music in the Festival of Orthodox Music in St Petersburg in 2012.
He had an extensive concert activity as a soloist singer in Cyprus, Italy and Russia. From 2018 he is choirmaster of the musicAeterna byzantina choir.